annotation of the m. tuberculosis hypothetical orfeome adding functional information to more than half of the uncharacterized proteins注释的结核分枝杆菌假设orfeome功能信息添加到超过一半的无特征的蛋白质.pdfVIP

Annotation of the M. tuberculosis Hypothetical Orfeome: Adding Functional Information to More than Half of the Uncharacterized Proteins 1 1 1 1,2 Tobias Doerks *, Vera van Noort , Pablo Minguez , Peer Bork 1 European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, 2 Max-Delbrueck-Centrum, Berlin, Germany Abstract The genome of Mycobacterium tuberculosis (H37Rv) contains 4,019 protein coding genes, of which more than thousand have been categorized as ‘hypothetical’ implying that for these not even weak functional associations could be identified so far. We here predict reliable functional indications for half of this large hypothetical orfeome: 497 genes can be annotated based on orthology, and another 125 can be linked to interacting proteins via integrated genomic context analysis and literature mining. The assignments include newly identified clusters of interacting proteins, hypothetical genes that are associated to well known pathways and putative disease-relevant targets. All together, we have raised the fraction of the proteome with at least some functional annotation to 88% which should considerably enhance the interpretation of large- scale experiments targeting this medically important organism. Citation: Doerks T, van Noort V, Minguez P, Bork P (2012) Annotation of the M. tuberculosis Hypothetical Orfeome: Adding Functional Information to More than Half of the Uncharacterized Proteins. PLoS ONE 7(4): e34302. doi:10.1371/journal.pone.0034302 Editor: Shoba Ranganathan, Macquarie University, Australia Received December 6, 2011; Accepted February 26, 2012; Published April 2, 2012 Copyright: 2012 Doerks et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits