有哪些信誉好的足球投注网站an integrated micro- and macroarchitectural analysis of the drosophila brain by computer-assisted serial section electron microscopy一个集成的微型和macroarchitectural分析果蝇大脑的计算机辅助连续切片电镜.pdfVIP
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an integrated micro- and macroarchitectural analysis of the drosophila brain by computer-assisted serial section electron microscopy一个集成的微型和macroarchitectural分析果蝇大脑的计算机辅助连续切片电镜
An Integrated Micro- and Macroarchitectural Analysis of
the Drosophila Brain by Computer-Assisted Serial
Section Electron Microscopy
1 2 2 3 4 4
Albert Cardona , Stephan Saalfeld , Stephan Preibisch , Benjamin Schmid , Anchi Cheng , Jim Pulokas ,
Pavel Tomancak2, Volker Hartenstein5,6*
¨ ¨
1 Institute of Neuroinformatics, ETH/University of Zurich, Zurich, Switzerland, 2 Max Plank Institute for Molecular Cell Biology and Genetics, Dresden, Germany, 3 Lehrstuhl
¨ ¨ ¨
fur Genetik und Neurobiologie, University of Wurzburg, Wurzburg, Germany, 4 Automated Molecular Imaging Group, The Scripps Research Institute (TSRI), San Diego,
California, United States of America, 5 Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, California, United
States of America, 6 Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America
Abstract
The analysis of microcircuitry (the connectivity at the level of individual neuronal processes and synapses), which is
indispensable for our understanding of brain function, is based on serial transmission electron microscopy (TEM) or one of
its modern variants. Due to technical limitations, most previous studies that used serial TEM recorded relatively small stacks
of individual neurons. As a result, our knowledge of microcircuitry in any nervous system is very limited. We applied the
software package TrakEM2 to reconstruct neuronal microcircuitry from TEM sections of a small brain, the early larval brain of
Drosophila melanogaster. TrakEM2 enables us to embed the analysis of the TEM image volumes at
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