an intronic signal for alternative splicing in the human genome可变剪接的intronic信号在人类基因组中.pdfVIP

an intronic signal for alternative splicing in the human genome可变剪接的intronic信号在人类基因组中.pdf

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an intronic signal for alternative splicing in the human genome可变剪接的intronic信号在人类基因组中

An Intronic Signal for Alternative Splicing in the Human Genome 1. 2. 3 2 4 4 5 2 Necat Havlioglu , Jun Wang , Kazuo Fushimi , Maria D. Vibranovski , Zhengyan Kan , Warren Gish , Alexei Fedorov , Manyuan Long *, Jane Y. Wu3* 1 Department of Pathology, Saint Louis University, St. Louis, Missouri, United States of America, 2 Department of Ecology and Evolution, The University of Chicago, Chicago, Illinois, United States of America, 3 Department of Neurology, Lurie Comprehensive Cancer Center, Center for Genetic Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America, 4 Department of Genetics, Washington University in St. Louis, St. Louis, Missouri, United States of America, 5 Department of Medicine and Program in Bioinformatics and Proteomics/ Genomics, Medical University of Ohio, Toledo, Ohio, United States of America An important level at which the expression of programmed cell death (PCD) genes is regulated is alternative splicing. Our previous work identified an intronic splicing regulatory element in caspase-2 (casp-2) gene. This 100-nucleotide intronic element, In100, consists of an upstream region containing a decoy 39 splice site and a downstream region containing binding sites for splicing repressor PTB. Based on the signal of In100 element in casp-2, we have detected the In100-like sequences as a family of sequence elements associated with alternative splicing in the human genome by using computational and experimental approaches. A survey of human genome reveals the presence of more than four thousand In100-like elements in 2757 genes. These In100-like elements tend to locate more frequent in intronic regions t

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