an inter-laboratory validation of a real time pcr assay to measure host excretion of bacterial pathogens, particularly of mycobacterium bovis的一个实验室内部验证实时pcr分析方法来衡量主机排泄的细菌病原体,特别是牛结核分枝杆菌.pdfVIP

an inter-laboratory validation of a real time pcr assay to measure host excretion of bacterial pathogens, particularly of mycobacterium bovis的一个实验室内部验证实时pcr分析方法来衡量主机排泄的细菌病原体,特别是牛结核分枝杆菌.pdf

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an inter-laboratory validation of a real time pcr assay to measure host excretion of bacterial pathogens, particularly of mycobacterium bovis的一个实验室内部验证实时pcr分析方法来衡量主机排泄的细菌病原体,特别是牛结核分枝杆菌

An Inter-Laboratory Validation of a Real Time PCR Assay to Measure Host Excretion of Bacterial Pathogens, Particularly of Mycobacterium bovis 1 1. 1 . 1 1 Emma R. Travis , William H. Gaze , Alessandra Pontiroli * , Francis P. Sweeney , David Porter , Sam 1 1 2 2 3 Mason , Matthew J. C. Keeling , Rebecca M. Jones , Jason Sawyer , Alicia Aranaz , Elena Castellanos 3 2 4 4 2 1 Rizaldos , Jennifer Cork , Richard J. Delahay , Gavin J. Wilson , R. Glyn Hewinson , Orin Courtenay , Elizabeth M. H. Wellington1 1 University of Warwick, School of Life Sciences, Coventry, United Kingdom, 2 Animal Health and Veterinary Laboratories Agency, Weybridge, United Kingdom, 3 School of Veterinary Sciences. Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department. Universidad Complutense de Madrid, Madrid, Spain, 4 Food and Environment Research Agency, Sand Hutton, York, United Kingdom Abstract Advances in the diagnosis of Mycobacterium bovis infection in wildlife hosts may benefit the development of sustainable approaches to the management of bovine tuberculosis in cattle. In the present study, three laboratories from two different countries participated in a validation trial to evaluate the reliability and reproducibility of a real time PCR assay in the detection and quantification of M. bovis from environmental samples. The sample panels consisted of negative badger faeces spik

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