altered desaturation and elongation of fatty acids in hormone-sensitive lipase null mice改变减饱和脂肪酸的伸长零小鼠皮下脂肪酶.pdfVIP

Altered Desaturation and Elongation of Fatty Acids in Hormone-Sensitive Lipase Null Mice ´ 1¤ 2 2 3 3 Celine Fernandez , Kai Schuhmann , Ronny Herzog , Barbara Fielding , Keith Frayn , Andrej 2 4 1 ¨ 1 Shevchenko , Peter James , Cecilia Holm , Kristoffer Strom * 1 Department of Experimental Medical Science, Lund University, Lund, Sweden, 2 Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, 3 Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, United Kingdom, 4 Department of Immunotechnology, Lund University, Lund, Sweden Abstract Background: Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored lipids, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrolyzes cholesteryl esters, retinyl esters and lipoidal esters. Despite its role in fatty acid mobilization, HSL null mice have been shown to be resistant to diet-induced obesity. The aim of this study was to define lipid profiles in plasma, white adipose tissue (WAT) and liver of HSL null mice, in order to better understand the role of this multifunctional enzyme. Methodology/Principal Findings: This study used global and targeted lipidomics and expression profiling to reveal changed lipid profiles in WAT, liver and plasma as well as altered expression of desaturases and elongases in WAT and liver of HSL null mice on high fat diet. Decreased mRNA levels of stearoyl-CoA desaturase 1 and 2 in