有哪些信誉好的足球投注网站abundance and genetic diversity of microbial polygalacturonase and pectate lyase in the sheep rumen ecosystem丰富和遗传多样性绵羊瘤胃微生物聚半乳糖醛酸酶和果胶酸裂解酶的生态系统.pdfVIP
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abundance and genetic diversity of microbial polygalacturonase and pectate lyase in the sheep rumen ecosystem丰富和遗传多样性绵羊瘤胃微生物聚半乳糖醛酸酶和果胶酸裂解酶的生态系统
Abundance and Genetic Diversity of Microbial
Polygalacturonase and Pectate Lyase in the Sheep
Rumen Ecosystem
. .
Peng Yuan , Kun Meng , Yaru Wang, Huiying Luo, Huoqing Huang, Pengjun Shi, Yingguo Bai,
Peilong Yang, Bin Yao*
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of
China
Abstract
Background: Efficient degradation of pectin in the rumen is necessary for plant-based feed utilization. The objective of this
study was to characterize the diversity, abundance, and functions of pectinases from microorganisms in the sheep rumen.
Methodology/Principal Findings: A total of 103 unique fragments of polygalacturonase (PF00295) and pectate lyase
(PF00544 and PF09492) genes were retrieved from microbial DNA in the rumen of a Small Tail Han sheep, and 66% of the
sequences of these fragments had low identities (,65%) with known sequences. Phylogenetic tree building separated the
PF00295, PF00544, and PF09492 sequences into five, three, and three clades, respectively. Cellulolytic and noncellulolytic
Butyrivibrio, Prevotella, and Fibrobacter species were the major sources of the pectinases. The two most abundant pectate
lyase genes were cloned, and their protein products, expressed in Escherichia coli, were characterized. Both enzymes
probably act extracellularly as their nucleotide sequences contained signal sequences, and they had optimal activities at the
ruminal physiological temperature and complementary pH-dependent activity profiles.
Conclusion/Significance: This study reveals the specificity, diversity, and abundance of pectinases in the rumen ecosystem
and provides two additional ruminal pectinases for potential industrial use under physiological conditions.
Citation: Yuan P,
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