a volumetric method for quantifying atherosclerosis in mice by using microct comparison to en face容积方法量化动脉粥样硬化小鼠通过microct相比在脸上.pdfVIP

A Volumetric Method for Quantifying Atherosclerosis in Mice by Using MicroCT: Comparison to En Face 1 1 2 2 3 4 David J. Lloyd *, Joan Helmering , Stephen A. Kaufman , James Turk , Matt Silva , Sergio Vasquez , 4 4 1 ´ 1 David Weinstein , Brad Johnston , Clarence Hale , Murielle M. Veniant 1 Department of Metabolic Disorders, Amgen Inc., Thousand Oaks, California, United States of America, 2 Department of Pathology, Amgen Inc., Thousand Oaks, California, United States of America, 3 Department of Research Imaging, Amgen Inc., Thousand Oaks, California, United States of America, 4 Numira Biosciences, Salt Lake City, Utah, United States of America Abstract Precise quantification of atherosclerotic plaque in preclinical models of atherosclerosis requires the volumetric assessment of the lesion(s) while maintaining in situ architecture. Here we use micro-computed tomography (microCT) to detect ex vivo aortic plaque established in three dyslipidemic mouse models of atherosclerosis. All three models lack the low-density lipoprotein receptor (Ldlr2/ 2), each differing in plaque severity, allowing the evaluation of different plaque volumes using microCT technology. From clearly identified lesions in the thoracic aorta from each model, we were able to determine plaque volume (0.04–3.1 mm3 2 ), intimal surface area (0.5–30 mm ), and maximum plaque (intimal-medial) thickness (0.1– 0.7 mm). Further, quantification of aortic volume allowed calculation of vessel occlusion by the plaque. To validate micr