a widespread distribution of genomic cemyod binding sites revealed and cross validated by chip-chip and chip-seq techniques广泛分布的基因组cemyod结合位点chip-chip和chip-seq技术揭示和交叉验证.pdfVIP

A Widespread Distribution of Genomic CeMyoD Binding Sites Revealed and Cross Validated by ChIP-Chip and ChIP-Seq Techniques 1 1 2 3 4 1 Haiyan Lei , Tetsunari Fukushige , Wei Niu , Mihail Sarov , Valerie Reinke , Michael Krause * 1 National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Department of Molecular, Cellular Developmental Biology, Yale University, New Haven, Connecticut, United States of America, 3 Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany, 4 Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America Abstract Identifying transcription factor binding sites genome-wide using chromatin immunoprecipitation (ChIP)-based technology is becoming an increasingly important tool in addressing developmental questions. However, technical problems associated with factor abundance and suitable ChIP reagents are common obstacles to these studies in many biological systems. We have used two completely different, widely applicable methods to determine by ChIP the genome-wide binding sites of the master myogenic regulatory transcription factor HLH-1 (CeMyoD) in C. elegans embryos. The two approaches, ChIP-seq and ChIP-chip, yield strongly overlapping results revealing that HLH-1 preferentially binds to promoter regions of genes enriched for E-box sequences (CANNTG), known binding sites for this well-studied class of transcription factors. HLH-1 binding sites were enriched upstream of genes known to be expressed in muscle, consistent with its role as a direct transcriptional regulator. HL