a simple and rapid method for dna isolation from xylophagous insects一个简单而快速的方法dna隔绝xylophagous昆虫.pdfVIP
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a simple and rapid method for dna isolation from xylophagous insects一个简单而快速的方法dna隔绝xylophagous昆虫
Int. J. Mol. Sci. 2010, 11, 5056-5064; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Technical Note
A Simple and Rapid Method for DNA Isolation from
Xylophagous Insects
Nancy Calderón-Cortés 1,*, Mauricio Quesada 1, Horacio Cano-Camacho 2 and
Guadalupe Zavala-Páramo 2
1 Center for Ecosystems Research (CIEco), National Autonomous University of Mexico (UNAM),
Morelia, Mexico; E-Mail: mquesada@oikos.unam.mx (M.Q.)
2 Multidisciplinary Center of Biotechnology Studies (CMEB), Michoacan University of San Nicolas
of Hidalgo (UMSNH), Morelia, Mexico; E-Mails: hcano1gz1@ (H.C.-C.);
gzavpar@ (G.Z.-P.)
* Author to whom correspondence should be addressed; E-Mail: ncalderon@oikos.unam.mx;
Tel./Fax: +52-(555)-6-23-27-19.
Received: 8 October 2010; in revised form: 22 November 2010 / Accepted: 30 November 2010 /
Published: 7 December 2010
Abstract: Published methods to isolate DNA from insects are not always effective in
xylophagous insects because they have high concentrations of phenolics and other
secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective
cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for
isolation of high quality DNA from xylophagous insects is described. This method
was successfully applied to PCR and restriction analysis, indicating removal
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