a two-staged model of na+ exclusion in rice explained by 3d modeling of hkt transporters and alternative splicingna +排斥在水稻的两阶段模型解释为3 d建模hkt转运蛋白和可变剪接.pdfVIP
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atwo-stagedmodelofnaexclusioninriceexplainedby3dmodelingofhkttransportersandalternativesplicingna排斥在水稻的两阶段模型解释为3d建模hkt转运蛋白和可变剪接
A Two-Staged Model of Na+ Exclusion in Rice Explained
by 3D Modeling of HKT Transporters and Alternative
Splicing
Olivier Cotsaftis*, Darren Plett, Neil Shirley, Mark Tester, Maria Hrmova
Australian Centre for Plant Functional Genomics, University of Adelaide, Adelaide, South Australia, Australia
Abstract
+ + +
The HKT family of Na and Na /K transporters is implicated in plant salinity tolerance. Amongst these transporters, the
cereal HKT1;4 and HKT1;5 are responsible for Na+ exclusion from photosynthetic tissues, a key mechanism for plant salinity
tolerance. It has been suggested that Na+ is retrieved from the xylem transpiration stream either in the root or the leaf
sheath, protecting the leaf blades from excessive Na+ accumulation. However, direct evidence for this scenario is scarce.
Comparative modeling and evaluation of rice (Oryza sativa) HKT-transporters based on the recent crystal structure of the
bacterial TrkH K+ transporter allowed to reconcile transcriptomic and physiological data. For OsHKT1;5, both transcript
abundance and protein structural features within the selectivity filter could control shoot Na+ accumulation in a range of
rice varieties. For OsHKT1;4, alternative splicing of transcript and the anatomical complexity of the sheath needed to be
taken into account. Thus, Na+ accumulation in a specific leaf blade seems to be regulated by abundance of a correctly
spliced OsHKT1;4 transcript in a corresponding sheath. Overall, allelic variation of leaf blade Na+ accumulation can be
explained by a complex interplay of gene transcription, alternative splicing and protein structure.
Citation: Cotsaftis O, Plett D, Shirley N, Tester M, Hrmova M (2012) A Two-Staged Mo
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