有哪些信誉好的足球投注网站a selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules选择性试验来检测甲壳素和生物活性纳米机器对其内在chitin-biosynthesis chitin-synthase分子.pdfVIP
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a selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules选择性试验来检测甲壳素和生物活性纳米机器对其内在chitin-biosynthesis chitin-synthase分子
Int. J. Mol. Sci. 2010, 11, 3122-3137; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
A Selective Assay to Detect Chitin and Biologically Active
Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic
Chitin-Synthase Molecules
Yury Herasimenka, Marta Kotasinska, Stefan Walter and Hildgund Schrempf *
FB Biology/Chemistry, Applied Genetics of Microorganisms, University Osnabrück, Barbarastr. 13,
D-49069 Osnabrück, Germany; E-Mails: herasimenka@ (Y.H.);
marta.kotasinska@gmx.de (M.K.); stefan.walter@biologie.uni-osnabrueck.de (S.W.)
* Author to whom correspondence should be addressed;
E-Mail: schrempf@biologie.uni-osnabrueck.de; Tel.: +49-541-969-2895; Fax: +49-541-969-2804.
Received: 22 June 2010; in revised form: 14 August 20 10 / Accepted: 26 August 2010 /
Published: 7 September 2010
Abstract: A new assay system for chitin has been developed. It comprises the
chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter
of which was chemically coupled to horseradish peroxidase. With the resulting complex,
minimal quantities of chitin are photometrically detectable. In addition, the assay allows
rapid scoring of the activity of chitin-synthases. As a result, a refined procedure for the
rapid purification of yeast chitosomes (nano-machineries for chitin biosynthesis) has been
establ
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