a preliminary analysis of the immunoglobulin genes in the african elephant (loxodonta africana)初步分析免疫球蛋白基因的非洲象(学名loxodonta africana).pdfVIP

A Preliminary Analysis of the Immunoglobulin Genes in the African Elephant (Loxodonta africana) 1 2 2 1 3 1 1,4 Yongchen Guo , Yonghua Bao *, Hui Wang , Xiaoxiang Hu , Zhihui Zhao , Ning Li , Yaofeng Zhao * 1 State Key Laboratory of AgroBiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People’s Republic of China, 2 Department of Basic Immunology, Xinxiang Medical University, Xinxiang, People’s Republic of China, 3 Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People’s Republic of China, 4 College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, People’s Republic of China Abstract The genomic organization of the IgH (Immunoglobulin heavy chain), Igk (Immunoglobulin kappa chain), and Igl (Immunoglobulin lambda chain) loci in the African elephant (Loxodonta africana) was annotated using available genome data. The elephant IgH locus on scaffold 57 spans over 2,974 kb, and consists of at least 112 VH gene segments, 87 DH gene segments (the largest number in mammals examined so far), six JH gene segments, a single m, a d remnant, and eight c genes (a and e genes are missing, most likely due to sequence gaps). The Igk locus, found on three scaffolds (202, 50 and 86), contains a total of 153 Vk gene segments, three Jk segments, and a single Ck gene. Two different transcriptional orientations were determined for these Vk gene segments. In contrast, the Igl locus on scaffold 68 includes 15 Vl gene segments, all with the same transcriptional polarity as the downstream Jl-Cl cluster. These data suggest t