a new system for the rapid collection of large numbers of developmentally staged zebrafish embryos新系统的快速收集大量发育了斑马鱼胚胎.pdfVIP

A New System for the Rapid Collection of Large Numbers of Developmentally Staged Zebrafish Embryos 1,2 5 6 1,2,3,4 Isaac Adatto , Christian Lawrence , Michael Thompson , Leonard I. Zon * 1 Stem Cell Program and Division of Hematology/Oncology, Children’s Hospital and Dana Farber Cancer Institute, Boston, Massachusetts, United States of America, 2 Harvard Stem Cell Institute, Boston, Massachusetts, United States of America, 3 Harvard Medical School, Boston, Massachusetts, United States of America, 4 Howard Hughes Medical Institute, Boston, Massachusetts, United States of America, 5 Aquatic Resources Program, Children’s Hospital Boston, Boston, Massachusetts, United States of America, 6 Plastics Concepts, Inc., Billerica, Massachusetts, United States of America Abstract The zebrafish is an excellent genetic and developmental model system used to study biology and disease. While the zebrafish model is associated with high fecundity, its reproductive potential has not been completely realized by scientists. One major issue is that embryo collection is inefficient. Here, we have developed an innovative breeding vessel designed to stimulate the natural reproductive behavior of the fish. This novel apparatus allows us to collect large numbers of developmentally synchronized embryos in brief and defined windows of time, and with minimal investments in labor and space. To demonstrate the efficacy of this approach, we placed three separate groups (n = 180) of fish in the vessel and allowed them to spawn for 10-minute intervals. During these trials, which were repeated three times, the fish produced 8600 6917, and 680061997 embryos, respectively. This