a non-vh1-69 heterosubtypic neutralizing human monoclonal antibody protects mice against h1n1 and h5n1 viruses一个非- vh1 - 69 heterosubtypic中和人类单克隆抗体能保护小鼠免受h1n1病毒和h5n1病毒.pdfVIP

A Non-VH1-69 Heterosubtypic Neutralizing Human Monoclonal Antibody Protects Mice against H1N1 and H5N1 Viruses 1. 1. 1. 1 1 Donata De Marco , Nicola Clementi , Nicasio Mancini , Laura Solforosi , Guisella J. Moreno , 2 2 2 2 1 Xiangjie Sun , Terrence M. Tumpey , Larisa V. Gubareva , Vasiliy Mishin , Massimo Clementi , Roberto Burioni1* 1 Laboratory of Microbiology and Virology, Vita-Salute San Raffaele University, Milan, Italy, 2 Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America Abstract Influenza viruses are among the most important human pathogens and are responsible for annual epidemics and sporadic, potentially devastating pandemics. The humoral immune response plays an important role in the defense against these viruses, providing protection mainly by producing antibodies directed against the hemagglutinin (HA) glycoprotein. However, their high genetic variability allows the virus to evade the host immune response and the potential protection offered by seasonal vaccines. The emergence of resistance to antiviral drugs in recent years further limits the options available for the control of influenza. The development of alternative strategies for influenza prophylaxis and therapy is therefore urgently needed. In this study, we describe a human monoclonal antibody (PN-SIA49) that recognizes a highly conserved epitope located on the stem region of the HA and able to neutralize a broad spectrum