a novel biochemical route for fuels and chemicals production from cellulosic biomass一种新的生化途径从纤维素生物质生产燃料和化学品.pdfVIP

A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass 1 1 1 2,3 1,4 1 Zhiliang Fan *, Weihua Wu , Amanda Hildebrand , Takao Kasuga , Ruifu Zhang , Xiaochao Xiong 1 Biological and Agricultural Engineering Department, University of California Davis, Davis, California, United States of America, 2 Department of Plant Pathology, University of California Davis, Davis, California, United States of America, 3 Agricultural Research Service, United States Department of Agriculture, Davis, California, United States of America, 4 College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangshu, People’s Republic of China Abstract The conventional biochemical platform featuring enzymatic hydrolysis involves five key steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as reactive intermediates for subsequent fermentation to fuels and chemicals. Herein, an alternative biochemical route is proposed. Pretreatment, enzymatic hydrolysis and cellulase production is consolidated into one single step, referred to as consolidated aerobic processing, and sugar aldonates are produced as the reactive intermediates for biofuels production by fermentation. In this study, we demonstrate the viability of consolidation of the enzymatic hydrolysis and cellulase production steps in the new route using Neurospora crassa as the model microorganism and the conversion of cellulose to ethanol as the model system. We intended to prove the two hypotheses: 1) cellulose can be directed to produce cellobionate by reducing b-glucosidase production an