a novel approach for automated analysis of cell attachment and spreading based on backscattered electron imaging by scanning electron microscopy自动化分析的新方法细胞附着和传播根据扫描电镜的背散射电子成像.pdfVIP

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a novel approach for automated analysis of cell attachment and spreading based on backscattered electron imaging by scanning electron microscopy自动化分析的新方法细胞附着和传播根据扫描电镜的背散射电子成像.pdf

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a novel approach for automated analysis of cell attachment and spreading based on backscattered electron imaging by scanning electron microscopy自动化分析的新方法细胞附着和传播根据扫描电镜的背散射电子成像

Materials 2009, 2, 1402-1416; doi:10.3390/ma2031402 OPEN ACCESS materials ISSN 1996-1944 /journal/materials Article A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy 1 1 1 1 2 Alisa Katsen-Globa , Leonora Peter , Susan Zöllner , Thomas Dörge , Martin Daffertshofer , Hartwig Preckel 2, Daniel Schmitt 1 and Heiko Zimmermann 1,* 1 Fraunhofer IBMT, Ensheimer Str.48, 66386 St.Ingbert, Germany; E-Mails: alisa.katsen@ibmt.fraunhofer.de (A.K.); leonora.peter@ibmt.fraunhofer.de (L.P.); susan.zoellner@ibmt.fraunhofer.de (S.Z.); thomas.doerge@ibmt.fraunhofer.de (T.D.); daniel.schmitt@ibmt.fraunhofer.de (D.S.) 2 Perkin Elmer Cellular Technologies GmbH, Schnackenburgallee 114, 22525 Hamburg, Germany; E-Mails: martin.daffertshofer@ (M.D.); hartwig.preckel@ (H.P.) * Author to whom correspondence should be addressed; E-Mail: heiko.zimmermann@ibmt.fraunhofer.de (H.Z.); Tel.: +49-6894-980-246; Fax: +49-6894-980-444. Received: 17 August 2009; in revised form: 21 September 2009 / Accepted: 23 September 2009 / Published: 24 September 2009 Abstract: The development of new materials for biological application requires in vitro testing of cell/surface interactions. Cell adhesion and spreading are difficult to quantify as most materials are non-transparent and transmission microscopy cannot be used. Contrast in reflection microscopy is r