a mechanism regulating the onset of sox2 expression in the embryonic neural plate发病的机制调节sox2表达在胚胎神经板.pdfVIP

PLoS BIOLOGY A Mechanism Regulating the Onset of Sox2 Expression in the Embryonic Neural Plate 1 2,3 2,3 4 1 4 Costis Papanayotou , Anne Mey , Anne-Marie Birot , Yasushi Saka , Sharon Boast , Jim C. Smith , 2,3 1* Jacques Samarut , Claudio D. Stern ´ ´ 1 Department of Anatomy Developmental Biology, University College London, London, United Kingdom, 2 Institut de Genomique Fonctionnelle de Lyon, Universite de ´ Lyon, Lyon, France, 3 Ecole Normale Superieure de Lyon, CNRS/INRA, Lyon, France, 4 Wellcome/Cancer Research UK Gurdon Institute for Cancer and Developmental Biology, Cambridge, United Kingdom In vertebrate embryos, the earliest definitive marker for the neural plate, which will give rise to the entire central nervous system, is the transcription factor Sox2. Although some of the extracellular signals that regulate neural plate fate have been identified, we know very little about the mechanisms controlling Sox2 expression and thus neural plate identity. Here, we use electroporation for gain- and loss-of-function in the chick embryo, in combination with bimolecular fluorescence complementation, two-hybrid screens, chromatin immunoprecipitation, and reporter assays to study protein interactions that regulate expression of N2, the earliest enhancer of Sox2 to be activated and which directs expression to the largest part of the neural plate. We show that interac