a mechanical model of cell segregation driven by differential adhesion细胞分离的机械模型由微分附着力.pdfVIP

A Mechanical Model of Cell Segregation Driven by Differential Adhesion 1 1 2 1 1 William R. Taylor *, Rosalind Morley , Alexey Krasavin , Lauren Gregory , David G. Wilkinson , Alexei Poliakov1 1 Division of Mathematical Biology and Division of Developmental Neurobiology, MRC National Institute for Medical Research, London, United Kingdom, 2 Department of Physics, King’s College London, London, United Kingdom Abstract From simulations that begin with a random mix of two cell types, we monitor progress towards segregation driven by contact-mediated linkage of model cells, which is equivalent to the cell-cell adhesion of real cells. In comparison with real cell experiments, we show that this mechanical model can account for the observed extent of segregation obtained by differential adhesion in a 2D cell culture assay of cells with differentially expressed cadherin molecules. Calibration of virtual to real time allowed us to estimate a time course for these experiments that was within 50% agreement for the simulations compared to differential adhesion of cells. In contrast, simulations of differential adhesion do not account for the rate of segregation driven by interactions between EphB2 receptor and ephrinB1 expressing cells which occurs an order of magnitude faster. The latter result suggests that mechanisms additional or alternative to differential adhesion contribute to Eph-ephrin mediated cell segregation. Citation: Taylor WR, Morley R, Krasavin A, Gregory L, Wilkinson DG, et al. (2012) A Mechanical Model of Cell Segregation Driven by Differential Adhesion. PLoS ONE 7(8): e43226. doi:10.1371/journal.pone.0043226 Editor: Frederic Andre, Aix-Marseille University, France Received March 27, 2012; Accepted J