细菌的简单染色法和革兰氏染色（Simple staining and Gram staining of bacteria）.docVIP

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细菌的简单染色法和革兰氏染色（Simple staining and Gram staining of bacteria）.doc

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细菌的简单染色法和革兰氏染色（Simple staining and Gram staining of bacteria）

细菌的简单染色法和革兰氏染色（Simple staining and Gram staining of bacteria） Actual address: 74-EA-3A-7A-55-D1 IP address: 169.254.8.242 Subnet mask: 255.255.0.0 Default gateway: DNS server: 59.54.76.83 WINS server: 1) experimental purpose: To study simple staining of bacteria and Gram staining method. (two) experimental principle: the dyes used for biological dyeing are mainly alkaline dyes, acid dyes and neutral dyes (1) the purpose of the experiment was to study the simple staining of bacteria and gram staining. (two) experimental principle: the dyes used for biological dyeing are mainly three kinds: basic dyes, acid dyes and neutral dyes. The ion of an alkali dye is positively charged and combines with a negatively charged substance. Due to bacterial protein isoelectric point is low, when it grows in neutral, alkaline or weak acidic solution often negatively charged, so we usually use basic dyes (such as methylene blue, crystal violet, magenta or green etc.) the coloring. The ion of an acid dye is negatively charged and can be combined with a positively charged substance. When the bacteria break down sugars and produce acid, the culture medium pH drops, and the positive charge increases with the bacteria, so it is easily colored by acidic dyes such as eosin, acid fuchsin or Congo red. Neutral dye is a combination of the former two also called composite dyes, such as eosin methylene blue and eosin azure etc.. Simple staining is the use of only one dye to stain bacteria to show their morphology. Simple staining does not identify the structure of bacterial cells. Gram staining was established in 1884 by C.Gram, a Danish pathologist. Gram staining can differentiate all bacteria into two groups: gram positive bacteria (G+) and gram negative bacteria (G -), which are the most commonly used differential methods in bacteriology. The staining method allows the bacteria to be divided into G+ bacteria and G - bacteria, determined by differences in the cell wall structure and composition