细菌形态与结构的检查法（Examination of bacterial morphology and structure）.docVIP

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细菌形态与结构的检查法（Examination of bacterial morphology and structure）.doc

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细菌形态与结构的检查法（Examination of bacterial morphology and structure）

细菌形态与结构的检查法（Examination of bacterial morphology and structure） Examination of bacterial morphology and structure [Objective] Traditional Chinese medicine (orthopedics and traumatology), acupuncture and massage [class hour] 3 hours First, the purpose of the experiment 1. Learn how to use and protect the microscope oil mirror. 2. Recognize the basic morphology and specific structure of bacteria (capsules, flagella, spores). 3. Master Gram staining method. 4. Understand the power of bacteria. Two 、 experimental principle 1, the use of microscope oil microscope principle: To observe the bacteria must be magnified about 1000 times the oil mirror. The lens of the oil mirror is very small, the focal length is the shortest, and the light passes through the glass and the air. The refraction of the medium is different, and the light of the human lens tube is very small, and the view field is dark and the image is not clear. As in the slide and the lens (lens) coupled between refractive index and glass (n=1.52) near cedar oil (n=1.515) can reduce the refraction of light, brightness strengthen vision, get a clear image. 2, the principle of Gram staining method: mainly gram positive bacteria and gram negative bacteria cell wall is different, in addition, gram positive bacteria isoelectric point than gram negative bacteria low, and alkaline dye affinity is strong. Three 、 instrument and equipment 1 microscope 2 bacterial specimens: (1) Staphylococcus (Gram staining), (2) Escherichia coli (Gram staining (3), Vibrio cholerae (Gram staining), (4) typhoid bacilli (flagella staining), (5) pneumococcus (capsule staining (6), tetanus bacillus (spore staining) Staphylococcus aureus and Pseudomonas aeruginosa agar 18 ~ 24 hours of culture. 4, liquid crystal violet staining and iodine, 95% alcohol, dilute carbolfuchsin. Four, related knowledge points Bacteria are unicellular prokaryotic microorganism of a class of small size, small and transparent living cells, when the bacteria suspended