neurite outgrowth of mature retinal ganglion cells and pc12 cells requires activity of ck1δ and ck1ε神经突的成熟视网膜神经节细胞和pc12细胞需要ck1δ活动和ck1ε.pdfVIP

Neurite Outgrowth of Mature Retinal Ganglion Cells and PC12 Cells Requires Activity of CK1d and CK1e 1 ¨ 2 ¨ 2 1 . 2,3 . Joachim Bischof , Adrienne Muller , Miriam Fander , Uwe Knippschild * , Dietmar Fischer * 1 Department of General, Visceral and Transplantation Surgery, University of Ulm, Ulm, Germany, 2 Department of Experimental Neurology, University of Ulm, Ulm, ¨ ¨ Germany, 3 Department of Experimental Neurology, University of Dusseldorf, Dusseldorf, Germany Abstract Mature retinal ganglion cells (RGCs) do not normally regenerate severed axons after optic nerve injury and show only little neurite outgrowth in culture. However, RGCs can be transformed into an active regenerative state after lens injury (LI) enabling these neurons to regrow axons in vitro and in vivo. In the current study we investigated the role of CK1d and CK1e activity in neurite outgrowth of LI stimulated RGCs and nerve growth factor (NGF) stimulated PC12 cells, respectively. In both cell types CK1d and e were localized in granular particles aligned at microtubules in neurites and growth cones. Although LI treatment did not measurably affect the expression of CK1d and e, it significantly elevated the specific kinase activity in the retina. Similarly, CK1d/e specific kinase activity was also elevated in NGF treated PC12 cells compared with untreated controls. Neurite extension in PC12 cells was associated with a change in the activity of CK1d C-terminal targeting kinases, suggesting that activity of the