neural stem cells achieve and maintain pluripotency without feeder cells神经干细胞达到和维持没有支线细胞多能性.pdfVIP

Neural Stem Cells Achieve and Maintain Pluripotency without Feeder Cells 1 1 1 1 1,2 3 Hyun Woo Choi , Jong Soo Kim , Sol Choi , Hyo Jin Jang , Min Jung Kim , Youngsok Choi , Hans R. ¨ 4 2 1 Scholer , Hyung Min Chung , Jeong Tae Do * 1 Department of Biomedical Science, Stem Cell and Developmental Biology, CHA University, Seoul, Republic of Korea, 2 CHA Bio and Diotech, Seoul, Republic of Korea, 3 Department of Biomedical Science, CHA University, Seoul, Republic of Korea, 4 Department of Cell and Developmental Biology, Max Planck Institute for Molecular ¨ Biomedicine, Munster, Germany Abstract Background: Differentiated cells can be reprogrammed into pluripotency by transduction of four defined transcription factors. Induced pluripotent stem cells (iPS cells) are expected to be useful for regenerative medicine as well as basic research. Recently, the report showed that mouse embryonic fibroblasts (MEF) cells are not essential for reprogramming. However, in using fibroblasts as donor cells for reprogramming, individual fibroblasts that had failed to reprogram could function as feeder cells. Methodology/Principal Finding: Here, we show that adult mouse neural stem cells (NSCs), which are not functional feeder cells, can be reprogrammed into iPS cells using defined four factors (Oct4, Sox2, Klf4, and c-Myc) under feeder-free conditions. The iPS cells, generated from NSCs expressing the Oct4-GFP reporter gene, could proliferate for more than two months (passage 20). Generated and maintained without feeder cells, these iPS cells expressed pluripotency markers (Oct4