near infrared imaging of egfr of oral squamous cell carcinoma in mice administered arsenic trioxide近红外成像egfr口腔鳞状细胞癌的小鼠注射三氧化二砷.pdfVIP

Near Infrared Imaging of EGFR of Oral Squamous Cell Carcinoma in Mice Administered Arsenic Trioxide 1. 2. 3 1 4 4 Lingbo Zhang , Kezheng Wang , Falin Zhao , Weiping Hu , Junjie Chen , Gregory M. Lanza , 2 1 Baozhong Shen *, Bin Zhang * 1 Stomatology Department, Institute of Hard Tissue Development and Regeneration, 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China, 2 Radiology Department and Molecular Imaging Center, 4th Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China, 3 School of Health Management, Hangzhou Normal University, Hangzhou, Zhejiang, China, 4 Division of Cardiology and C-TRAIN, Washington University School of Medicine, St. Louis, Missouri, United States of America Abstract Background: The effectiveness of near-infrared imaging (NIR) interrogation of epidermal growth factor receptor (EGFR) expression as a sensitive biomarker of oral squamous cell carcinoma (OSCC) response to arsenic trioxide therapy was studied in mice. Material and Methods: A431 OSCC in vitro were exposed to 0 mM, 0.5 mM, 2.5 mM, or 5 mM of As O for 0 h, 24 h, 48 h and 2 3 72 h. Confocal microscopy and flow cytometry confirmed EGFR expression and demonstrated a sensitivity dose-related signal decline with As O treatment. Next, mice with pharynx-implanted A431 cells received As O i.p. every 48 h at 0.0, 0.5, 2 3