movement protein pns6 of rice dwarf phytoreovirus has both atpase and rna binding activities运动蛋白pns6水稻矮phytoreovirus atp酶和核糖核酸绑定活动.pdfVIP

Movement Protein Pns6 of Rice dwarf phytoreovirus Has Both ATPase and RNA Binding Activities 1. 1. 1 2 3 4 4 1 Xu Ji , Dan Qian , Chunhong Wei , Gongyin Ye , Zhongkai Zhang , Zujian Wu , Lianhui Xie , Yi Li * 1 State Key Laboratory of Protein and Plant Gene Research, Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, Peking University, Beijing, People’s Republic of China, 2 State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, People’s Republic of China, 3 Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, People’s Republic of China, 4 Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, People’s Republic of China Abstract Cell-to-cell movement is essential for plant viruses to systemically infect host plants. Plant viruses encode movement proteins (MP) to facilitate such movement. Unlike the well-characterized MPs of DNA viruses and single-stranded RNA (ssRNA) viruses, knowledge of the functional mechanisms of MPs encoded by double-stranded RNA (dsRNA) viruses is very limited. In particular, many studied MPs of DNA and ssRNA viruses bind non-specifically ssRNAs, leading to models in which ribonucleoprotein complexes (RNPs) move from cell to cell. Thus, it will be of special interest to determine whether MPs of dsRNA viruses interact with genomic dsRNAs or their derivative sRNAs. To this end, we studied the biochemical functions of MP Pns6 of Rice dwarf phytoreovirus (RDV), a member of Phytoreovirus that contains a 12-segmented dsRNA genome. We report here that Pns6 binds b