nedd9 is a positive regulator of epithelial-mesenchymal transition and promotes invasion in aggressive breast cancernedd9 epithelial-mesenchymal过渡是一个积极监管机构和促进在侵袭性乳腺癌中入侵.pdfVIP

NEDD9 Is a Positive Regulator of Epithelial-Mesenchymal Transition and Promotes Invasion in Aggressive Breast Cancer 1 2 3 2 3 4 1 Chenfei Kong , Changqing Wang , Liping Wang , Musong Ma , Chunbo Niu , Xiaoqian Sun , Juan Du , 1 1 4 1 Zhixiong Dong , Shan Zhu , Jun Lu *, Baiqu Huang * 1The Institute of Genetics and Cytology, Northeast Normal University, Changchun, People’s Republic of China, 2 The Breast Surgery, The Tumor Hospital of Jilin Province, Changchun, People’s Republic of China, 3 The Pathology Department, The Bethune Hospital of Jilin University, Changchun, People’s Republic of China, 4 The Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, People’s Republic of China Abstract Epithelial to mesenchymal transition (EMT) plays an important role in many biological processes. The latest studies revealed that aggressive breast cancer, especially the triple-negative breast cancer (TNBC) subtype was frequently associated with apparent EMT, but the mechanisms are still unclear. NEDD9/HEF1/Cas-L is a member of the Cas protein family and was identified as a metastasis marker in multiple cancer types. In this study, we wished to discern the role of NEDD9 in breast cancer progression and to investigate the molecular mechanism by which NEDD9 regulates EMT and promotes invasion in triple-negative breast cancer. We showed that expression of NEDD9 was frequently upregulated in TNBC cell lines, and in aggressive breast tumors, especially in TNBC subtype. Knockdown of endogenous NEDD9 reduced the migration, invasion and proliferation of TNBC cells. Moreover, ectopic overexpression of NEDD9 in mamma