mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry模具在临床实验室常规识别matrix-assisted激光解吸电离飞行时间质谱分析.pdfVIP

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2017-09-01 发布于上海
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mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry模具在临床实验室常规识别matrix-assisted激光解吸电离飞行时间质谱分析.pdf

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mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry模具在临床实验室常规识别matrix-assisted激光解吸电离飞行时间质谱分析

Mould Routine Identification in the Clinical Laboratory by Matrix-Assisted Laser Desorption Ionization Time-Of- Flight Mass Spectrometry 1 ´ 1 ´ 1 2 1 Carole Cassagne *, Stephane Ranque , Anne-Cecile Normand , Patrick Fourquet , Sandrine Thiebault , 3 3 1 Chantal Planard , Marijke Hendrickx , Renaud Piarroux ´ ´ ´ ´ 1 Laboratoire de Parasitologie-Mycologie, CHU Timone, Universite de la Mediterranee, Marseille, France, 2 Service Proteomique, Centre d’Immunologie de Marseille Luminy, Marseille, France, 3 IHEM: Scientific Institute of Public Health, Mycology and Aerobiology Section, Brussels, Belgium Abstract Background: MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts and revolutionized the daily clinical laboratory routine. But it has not been established for routine mould identification. This study aimed to validate a standardized procedure for MALDI-TOF MS-based mould identification in clinical laboratory. Materials and Methods: First, pre-extraction and extraction procedures were optimized. With this standardized procedure, a 143 mould strains reference spectra library was built. Then, the mould isolates cultured from sequential clinical samples were prospectively subjected to this MALDI-TOF MS based-identification assay. MALDI-TOF MS-based identification was considered correct if it was concordant with the phenotypic identification; otherwise, the gold standard was DNA sequence comparison-based identification. Results: The optimized procedure comprised a culture on sabouraud-ge