mycobacterium tuberculosis complex mycobacteria as amoeba-resistant organisms结核分枝杆菌复杂的分枝杆菌amoeba-resistant生物.pdfVIP

Mycobacterium tuberculosis Complex Mycobacteria as Amoeba-Resistant Organisms 1,2 1 2 1 1 Felix Mba Medie , Iskandar Ben Salah , Bernard Henrissat , Didier Raoult , Michel Drancourt * ´ ´ ´ ´ ´ ´ 1 Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 6236 CNRS - Universite de la Mediterranee, IRD 189, IFR 48, Faculte de Medecine, ´ ´ ´ ´ Marseille, France, 2 Architecture et Fonction des Macromolecules Biologiques, AFMB, UMR 6098 CNRS - Universite de la Mediterranee, Marseille, France Abstract Background: Most environmental non-tuberculous mycobacteria have been demonstrated to invade amoebal trophozoites and cysts, but such relationships are largely unknown for members of the Mycobacterium tuberculosis complex. An environmental source has been proposed for the animal Mycobacterium bovis and the human Mycobacterium canettii. Methodology/Principal Findings: Using optic and electron microscopy and co-culture methods, we observed that 8960.6% of M. canettii, 12.4 60.3% of M. tuberculosis, 11.7 62% of M. bovis and 11.2 60.5% of Mycobacterium avium control organisms were phagocytized by Acanthamoeba polyphaga, a ratio significantly higher for M. canettii (P = 0.03), correlating with the significantly larger size of M. canetti organisms (P = 0.035). The percentage of intraamoebal mycobacteria surviving into cytoplasmic vacuoles was 3262% for M. canettii, 26 61% for M. t