identification of cancer cell-line origins using fluorescence image-based phenomic screening使用荧光图像识别癌症细胞系起源phenomic筛选.pdfVIP

Identification of Cancer Cell-Line Origins Using Fluorescence Image-Based Phenomic Screening 1 2 3 4 4 4 Jun-Seok Lee , Yun Kyung Kim , Han Jo Kim , Siti Hajar , Yee Ling Tan , Nam-Young Kang , Shin Hui 4 1 3,4 Ng , Chang No Yoon , Young-Tae Chang * 1 Biomolecules Function Research Center, Korea Institute of Science and Technology, Seoul, South Korea, 2 Center for Neuro-Medicine, Brain Science Institute, Korea Institute of Science and Technology, Seoul, South Korea, 3 Department of Chemistry Med Chem Program, Life Sciences Institute, National University of Singapore, Singapore, Singapore, 4 Lab of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore, Singapore Abstract Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCI’s 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluorescence intensity analysis, we successfully discriminated the organ origin of all the 60 cell-lines. Citation: Lee J-S, Kim YK, Kim HJ, Hajar S, Tan YL, et al. (2012) Identification of Cancer Cell-Line Origins Using Fluorescence Image-Based Phenomic Screening. PLoS ONE 7(2): e32096. doi:10.1371/journal.pone.0032096 Editor: Nikos K. Karamanos, University of Patras, Greece Received October 5, 2011; Accepted January 19, 2012; Published February 23, 2012 Copyright: 2012 Lee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits u