identification and characterization of a new orthoreovirus from patients with acute respiratory infections识别和描述一个新的orthoreovirus从急性呼吸道感染患者.pdfVIP

Identification and Characterization of a New Orthoreovirus from Patients with Acute Respiratory Infections 1,2 2 3 4 1 3 Kaw Bing Chua *, Kenny Voon , Gary Crameri , Hui Siu Tan , Juliana Rosli , Jennifer A. McEachern , 4 3 3 Sivagami Suluraju , Meng Yu , Lin-Fa Wang * 1 National Public Health Laboratory, Sg. Buloh, Selangor, Malaysia, 2 International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia, 3 CSIRO Livestock Industries, Australian Animal Health Laboratory and Australian Biosecurity Cooperative Research Center for Emerging Infectious Diseases, Geelong, Australia, 4 Klinik Kesihatan Kampar, Jalan Degong, Perak, Malaysia Abstract First discovered in the early 1950s, reoviruses (respiratory enteric orphan viruses) were not associated with any known disease, and hence named orphan viruses. Recently, our group reported the isolation of the Melaka virus from a patient with acute respiratory disease and provided data suggesting that this new orthoreovirus is capable of human-to-human transmission and is probably of bat origin. Here we report yet another Melaka-like reovirus (named Kampar virus) isolated from the throat swab of a 54 year old male patient in Kampar, Perak, Malaysia who was suffering from high fever, acute respiratory disease and vomiting at the time of virus isolation. Serological studies indicated that Kampar virus was transmitted from the index case to at least one other individual and caused respiratory disease in the contact case. Sequence analysis of the four small class genome segments indicated that Kampar and Melaka viruses are