honey bee pten – description, developmental knockdown, and tissue-specific expression of splice-variants correlated with alternative social phenotypes蜜蜂ptenu2014u2014描述、发展击倒,和组织表达splice-variants与替代社会表型.pdfVIP

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honey bee pten – description, developmental knockdown, and tissue-specific expression of splice-variants correlated with alternative social phenotypes蜜蜂ptenu2014u2014描述、发展击倒,和组织表达splice-variants与替代社会表型.pdf

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honey bee pten – description, developmental knockdown, and tissue-specific expression of splice-variants correlated with alternative social phenotypes蜜蜂ptenu2014u2014描述、发展击倒,和组织表达splice-variants与替代社会表型

Honey Bee PTEN – Description, Developmental Knockdown, and Tissue-Specific Expression of Splice- Variants Correlated with Alternative Social Phenotypes 1 ¤ 1 1 1,2 Navdeep S. Mutti * , Ying Wang , Osman Kaftanoglu , Gro V. Amdam 1 School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, 2 Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, Aas, Norway Abstract Background: Phosphatase and TENsin (PTEN) homolog is a negative regulator that takes part in IIS (insulin/insulin-like signaling) and Egfr (epidermal growth factor receptor) activation in Drosophila melanogaster. IIS and Egfr signaling events are also involved in the developmental process of queen and worker differentiation in honey bees (Apis mellifera). Here, we characterized the bee PTEN gene homologue for the first time and begin to explore its potential function during bee development and adult life. Results: Honey bee PTEN is alternatively spliced, resulting in three splice variants. Next, we show that the expression of PTEN can be down-regulated by RNA interference (RNAi) in the larval stage, when female caste fate is determined. Relative to controls, we observed that RNAi efficacy is dependent on the amount of PTEN dsRNA that is delivered to larvae. For larvae fed queen or worker diets containing a high amount of PTEN dsRNA, PTEN knockdown was significant at a whole-body level but lethal. A lower dosage did not result in a significant gene down-regulation. Finally, we compared same-aged adult workers with different behavior: nursing vs. foraging. We show that between nurses and foragers, PTEN isoforms were differentially expressed within brain, ovary and fat body tissues. All i