a novel escherichia coli o157h7 clone causing a major hemolytic uremic syndrome outbreak in china一种新型大肠杆菌o157h7克隆会引起溶血性尿毒症综合征疫情在中国.pdfVIP

A Novel Escherichia coli O157:H7 Clone Causing a Major Hemolytic Uremic Syndrome Outbreak in China Yanwen Xiong1., Ping Wang1., Ruiting Lan2., Changyun Ye1., Hua Wang3., Jun Ren4., Huaiqi Jing1., 1 5 1 1 1 1 1 Yiting Wang , Zhemin Zhou , Xuemei Bai , Zhigang Cui , Xia Luo , Ailan Zhao , Yan Wang , 1 1 5 1 Shaomin Zhang , Hui Sun , Lei Wang , Jianguo Xu * 1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, China, 2 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia, 3 Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu Province, China, 4 Anhui Provincial Center for Disease Control and Prevention, Hefei, Anhui Province, China, 5 College of Life Sciences, Nankai University, Tianjin, China Abstract An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level o