a key role of dendritic cells in probiotic functionality在益生菌树突状细胞功能的一个关键的角色.pdfVIP

A Key Role of Dendritic Cells in Probiotic Functionality 1,2 1 1 3 4 2 1 Benoit Foligne , Georgia Zoumpopoulou , Joelle Dewulf , Amena Ben Younes , Fabrice Chareyre , Jean-Claude Sirard , Bruno Pot , Corinne Grangette1* ´ ´ 1 Laboratoire de Bacteries Lactiques et Immunite des Muqueuses, Institut Pasteur de Lille - Institut de Biologie de Lille, Lille, France, 2 Institut National ´ ´ de la Sante et de la Recherche Medicale (INSERM)U801, Institut Pasteur de Lille - Institut de Biologie de Lille, Lille, France, 3 IFR142, Institut Pasteur de ´ ´ Lille - Institut de Biologie de Lille, Lille, France, 4 Institut National de la Sante et de la Recherche Medicale (INSERM) U674, Fondation Jean Dausset/ CEPH, Paris, France Background. Disruption of the intestinal homeostasis and tolerance towards the resident microbiota is a major mechanism involved in the development of inflammatory bowel disease. While some bacteria are inducers of disease, others, known as probiotics, are able to reduce inflammation. Because dendritic cells (DCs) play a central role in regulating immune responses and in inducing tolerance, we investigated their role in the anti-inflammatory potential of probiotic lactic acid bacteria. Methodology/Principal Findings. Selected LAB strains, while efficiently taken up by DCs in vitro, induced a partial maturation of the cells. Transfer of probiotic-treated DCs conferred protection against 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Protection was associated with a reduction of inflammato