a key role for poly(adp-ribose) polymerase 3 in ectodermal specification and neural crest development保利(adp-ribose)聚合酶3的一个关键的角色在外胚层的规范和神经嵴的发展.pdfVIP

A Key Role for Poly(ADP-Ribose) Polymerase 3 in Ectodermal Specification and Neural Crest Development ` 1,2 3 ´ 4 4 3 Michele Rouleau , Vishal Saxena , Amelie Rodrigue , Eric R. Paquet , Abbie Gagnon , Michael J. 5 2,4 3 1,2 Hendzel , Jean-Yves Masson , Marc Ekker , Guy G. Poirier * ´ ´ 1 Cancer Research Laboratory, CHUQ Research Center, Centre Hospitalier de l’Universite Laval, Quebec, Canada, 2 Department of Molecular Biology, Medical Biochemistry ´ and Pathology, Faculty of Medicine, Laval University, Quebec, Canada, 3 Center for Advanced Research in Environmental Genomics, Department of Biology, University of ˆ ´ ´ Ottawa, Ottawa, Canada, 4 Genome Stability Laboratory, Laval University Cancer Research Center, Hotel-Dieu de Quebec, Quebec, Canada, 5 Department of Oncology, Faculty of Medicine, University of Alberta and Cross Cancer Institute, Edmonton, Canada Abstract Background: The PARP family member poly(ADP-ribose) polymerase 3 (PARP3) is structurally related to the well characterized PARP1 that orchestrates cellular responses to DNA strand breaks and cell death by the synthesis of poly(ADP- ribose). In contrast to PARP1 and PARP2, the functions of PARP3 are undefined. Here, we reveal critical functions for PARP3 during vertebrate development. Principal Findings: We have used several in vitro and in vivo approache