微波-酶法提取远志总皂苷工艺探究.docVIP

微波-酶法提取远志总皂苷工艺探究摘 要:目的:优选远志总皂苷的微波-酶法最佳提取工艺条件。方法:用乙醇和水为溶剂,以远志提取液中总皂苷提取物的收率为指标,采用3因素3水平的正交设计法和单因素法对微波-复合酶法提取工艺条件进行优选。结果:实验条件下的最优工艺条件为:在pH=7,45℃条件下,酶解时间为1h,控制料液比1∶25,微波功率255W,提取4min,乙醇浓度95%,酶浓度为0.30%,远志总皂苷的提取率达2.03%;用显微镜观察到微波、复合酶破坏了远志细胞壁的结构;薄层色谱分析表明微波未破坏皂苷结构。结论:与直接传统乙醇回流提取法相比,微波-酶法提取能大大缩短提取时间,降低有机溶剂用量,并能提高远志总皂苷的收率。 关键词:微波-酶提取法;远志;总皂苷;正交实验 中图分类号:R284.2 文献标识码:A 文章编号:1673-7717(2010)01-0097-04 Microwave?enzymatic Extraction Process of Total Saponins in Polygala WANG Ying?li,WANG Yun?ting (Shanxi University of Traditional Chinese Medicine, Taiyuan 030024 ,Shanxi,China) Abstract:Objective:Optimization the extraction conditions of total saponins in Polygala using microwave?enzymatic extraction method.Methods:Using ethanol and water as solvent , the yields of total saponins in Polygala as indicator, using three factors and three levels of the orthogonal design and single-factor method of microwave?enzymatic extraction method, the extraction process conditions were optimized.Results:Under the experimental conditions the optimum process conditions are as follows: pH = 7,45 ℃, 1h hydrolysis time, the ratio ofmaterial to liquid is 1:25, microwave power is 255W, extraction time is 4min, the concentration of ethanol is 95% , enzyme concentration is 0.30%, the total extraction rate of total saponins in Polygala is 2.03%; From the microscope view the microwave and enzyme destroyed the cell wall of polygala; through the thin-layer chromatography plate, the microwave did not damaged the structure of saponins. Conclusion: Comparing to traditional method of direct ethanol extraction, microwave - enzymatic extraction method can greatly shorted the extraction time, reduce the amount of organic solvents and can improve the yields of total saponins in Polygala. Key words:microwave?enzymatic extraction method;Polygala;total saponins;orthogonal experiment 远志科远志属植物全世界有500多种,分布于欧亚大陆和美洲的亚热带和温带地区。我国有42种8变种,资源较丰富[1]。远志为常用中药之一,药用历史悠久,在《神农本草经》中列为上品。《中国药典》2005年版一部规定其来源为远志(