毛白杨生长素载体蛋白基因PtAUX1超表达的研究 - 中国农学通报.PDF

中国农学通报 2012,28(16):42-46 Chinese Agricultural Science Bulletin 毛白杨生长素载体蛋白基因PtAUX1超表达的研究 于淑惠，闫 辉，左曼曼，郎庆雯，樊金会 （山东农业大学林学院，山东泰安271018 ） 摘 要：以毛白杨和拟南芥为研究对象，研究生长素信号转导因子基因在植物发育过程中的作用。利用 构建好的PtAUX 1 的表达载体，转化拟南芥和毛白杨，获得转基因植株，通过PCR 技术检测转化植株，分 析植株表型并观察植株离体条件下的形态变化。结果显示PtAUX 1影响植株各器官的形态发育，毛白 杨长势旺盛、外植体有莲座状幼叶产生，拟南芥出现了匍匐生长、不改变根的向地性、茎上的表皮毛增 多、花器官畸形等现象。以上结果表明PtAUX 1基因通过生长素极性运输，促进各细胞、组织对内源生 长素IAA 的吸收，进而参与器官极性的建立，影响各器官的形态和发育过程。 关键词：PtAUX 1基因；毛白杨；拟南芥；生长素 中图分类号：S7 文献标志码：A 论文编号：2012-0504 TheStudyofOver-expressionoftheAuxinInfluxCarrierGenePtAUX1inPopulustomentosaCarr. Yu Shuhui, Yan Hui, Zuo Manman, Lang Qingwen, Fan Jinhui (CollegeofForestry,ShandongAgriculturalUniversity, Tai ’an Shandong 271018) Abstract: The aim was to study the action of genes encoding auxin signaling components in plants development. P. tomentosa and Arabidopsis were chosen as the research object in the paper. PtAUX 1 complied with the role of auxin characteristics and influenced organ morphology through the regulation of auxin polar transport in the distribution of the plants. By using expression vector of P. tomentosa PtAUX 1 constructed by our laboratory, the auxin influx import carrier PtAUX 1 was transformed into Arabidopsis and Poplar. PtAUX 1 transgenic plants were detected by PCR, and the phenotypes of transgenic plants and morphologic characters cultured invivowere analyzed. The experiments showed that the over-expression of PtAUX 1 influenced many organs of the morphological development. Transgenic plant of Poplar had vigorous growth and spire. Transgenic plant of Arabidopsis had grown in groveling , not altered root geotropism, trichome number increased on the stem, and abnorm