培养基的制备技术（Preparation technology of culture medium）.docVIP

培养基的制备技术（Preparation technology of culture medium） I. cleaning of glassware In the preparation of culture medium, we should use some glassware, such as test tube, triangle bottle, culture dish, beaker and straw. Before using these utensils, they should be washed and cleaned according to different conditions. Some should be packaged and sterilized before they can be used. 1, newly purchased glassware Remove the dirt contaminated with the package, first with hot soapy water scrub, rinse clean, and then soaked in 1 to 2% of the industrial hydrochloric acid for several hours, so that free alkaline substances removed, and then rinse with water. For the larger capacity of the vessel, such as a large flask, etc., wash after injection of concentrated hydrochloric acid with the internal surface of the container rotates equitably with hydrochloric acid, after a few minutes to pour hydrochloric acid, to rinse water, washing water, stand upside down on the air dry, you can use. 2. Used glassware Where there is no pathogenic bacteria or fungi have not been contaminated with utensils after use can wash, draw a Straw chemical reagent, can be soaked in water until after a certain number of concentrated cleaning. Containers contaminated by pathogens may be removed, removed by dirt, washed with soap, and rinsed with running water. If the soap can not be washed containers, can be soaked lotion, after proper time and then rinse with water. The main ingredients of the lotion are potassium dichromate and concentrated acid, which is used to oxidize organic substances into soluble substances for flushing. Lotion has a strong corrosive effect. Use it with great care to avoid splashing on clothes, body or other items. Two. The type of culture medium Any nutrient matrix prepared in the laboratory for microbial growth, reproduction, or accumulation of metabolites is called a culture medium (Media). Because of the different nutritional requirements of different microorganisms, the purpose of cu