拟环纹豹蛛pardosa pseudoannulata 卵黄蛋白的分离纯化及性质.pdfVIP

19 卷 5 期 生命科学研究 灾燥造援员9 晕燥援5 圆园15 年 10 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Oct. 圆园15 拟环纹豹蛛Pardosa p seudoannulata 卵黄蛋白 的分离纯化及性质 瑶, 黄 婷, 谭昭君, 颜亨梅* (湖南师范大学 生命科学学院, 中国湖南 长沙4 10081) 摘 要: 用蒸馏水沉淀法 Sephacryl S-300 High Resolution Hiprep 26/60 葡聚糖凝胶层析 蛋白质电泳的方法 提取和纯化了拟环纹豹蛛Pardos a p se udoannulata 的卵黄蛋白, 并对其性质进行了分析遥 用蒸馏水沉淀法能得 到卵黄蛋白粗提液, 将得到的卵黄蛋白粗提液用Sephacryl S-300 High Resolution Hiprep 26/60 凝胶柱过滤, 可 以得到纯化的卵黄蛋白遥 电泳结合不同的染色方法证明拟环纹豹蛛卵黄蛋白是一种糖脂复合蛋白, 它由2 个 亚基组成, 亚基的相对分子质量分别为80.1 kD 和57.4 kD 遥 关键词: 拟环纹豹蛛; 卵黄蛋白; 分离纯化 中图分类号: Q492.5 文献标识码: A 文章编号: 1007-7847(20 15)05-0392-05 Purification and Characterization of Vitellin from Pardosa p seudoannulata GONG Yao, HUANG Ting, TAN Zhao-jun, YAN Heng-mei* (College of L ife Sciences, H unan Normal Univers ity, Changsha 410081, H unan, China) Abstract: The vitellin was purified from eggs of the Pardosa p seudoannulata using distilled water precipita tion, sephadex gel filtration and gel electrophoresis. The crude yolk protein was obtained by precipitating with distilled water. The crude york protein was purified by Sephacryl S -300 High Resolution 26/60 Hiprep gel filtration. Its properties were also analyzed using polyacrylamide gel dlectrophoresis and differ ent dyeing methods. The results indicated that the vitellin of Pardos a p seudoannulata was a kind of protein containing sugar and fat, and such a protein mocule consists of two subunits with the relative molecular mass of 80.1 kD and 57.4 kD. Key words: Pardosa p seudoannulata; vitellin; purification