Ras-MAPK信号转导系统在醛固酮诱导心室成纤维细胞增殖中的作用.pdfVIP

第25卷 第1期 河 北 职 工 医 学 院 学 报 Vo1．25 No．1 2 0 0 8年2月 Joumal of Hebei Medical College for Continuing Education Feb． 20o8 · 临床医学· Ras．—． MAPK信号转导系统在醛固酮诱导 心室成纤维细胞增殖中的作用 冯惠平 ，陈春惠 。尹博英 。孙贺英 。潘涣军 。冯惠清 ，崔 炜 (1．河北大学附属医院，河北保定 071000；2．河北大学医学部，河北保定 071000；3．河北医科大学第二医院 河北石家庄 050071) 摘要：目的探讨醛固酮诱导心肌成纤维细胞(c )增殖的信号转导机制。方法用胰酶消化法分离、培养新生大鼠CFs，采用 H 一 胸腺嘧淀核苷( H—TdR)掺人法、Western—blotting技术从DNA合成、蛋白表达等方面观察MAPK活化与醛固酮促CFs增殖 的关系。结果(1)醛固酮剂量依赖性促CFsDNA合成，其作用可被PD98059逆转；(2)醛固酮对MAPK蛋白表达有显著增强作 用，此作用可被PD98059阻断。结论醛固酮能激活CFs的MAPK，MAPK表达及活化参与醛固酮诱导的CFS增殖。 关键词：醛固酮；心肌成纤维细胞；丝裂原活化蛋白激酶；细胞增殖 中图分类号：R一332 文献标识码：A 文章编号：1008—6692(2008)01—0015—03 Ras一～ LPK signa~transduction mechanisms in the inhibitive effects on cardiac fibroblasts proliferation in neonatl rat induced by aldosteronce Feng Huiping Chen Chunhui Ying Boying et．a1． (Affiliated Hospital ofHebei University，Baoding 071000，China) Abstract：Objective To provide experimental evidence for Ras——MAPK signal regulating cardiac fibroblasts proliferation induced by aldosteron．Methods：The DNA synthesis of cardiac fibroblasts was determined with H—Thymidine( H— TdR)incorporation，Western blotting analysis Was used detect phosphorylated MAPK protein expression．Results：1．Aldo sterone promoted the proliferation of cardiac fibroblasts in a dose dependent manner，PD98059 in concentration of 10～5 mol／L[(782±152)counts·min ]reversed the effects of 10。mol／L aldosterone[(1879±169)counts·min一 ]．2． Taken serum—free DMEM as control group(OD score=1)，10 mol／L aldosterone increased significantly the levels of the activated(phosphorylated)MAPK protein，and the levels of peak of activity of phospho—MAPK(8．11±0．46 fold at 4 hours)．Aldosterone with a concentration of 10 mol／L increased significantly the phosphorylated MAPK protein levels at 4 hours(8．11±0．46)。while PD98059 in a concentr