地尔硫卓对急性心肌梗死后大鼠心肌炎症的干预研究.pdfVIP

刘 英等 地尔硫卓对急性心肌梗死后大鼠心肌炎症的干预研究 第7期 · 临床免疫学 · 地尔硫卓对急性心肌梗死后大鼠心肌炎症的干预研究① 刘 英 廖玉华 程 翔 (华中科技大学同济医学院心血管病研究所附属协和医院心内科，武汉430022) 中国图书分类号 R542．2 文献标识码 A 文章编号 1000-484x(2007)07-0653-03 [摘 要] 目的：探讨地尔硫卓对大鼠急性心肌梗死(AMI)后心室重塑和心肌炎症反应的影响。方法：建立大鼠AMI模 型，存活鼠随机分为地尔硫卓组(D组)和对照组(AMI组)，另设假手术组(S组)。分别在梗死后1天、3天、1周、2周和4周 测量各组大鼠超声心动图变化、HE染色检测心肌炎症细胞浸润及半定量RT—PCR检测炎症因子的表达。结果：与s组比较， AMI组射血分数下降和左室重量增加，心肌组织炎症细胞浸润，致炎因子TNF— 、IL一1p、IL-6表达增多(P0．05)，而D组射 血分数和左室重量明显改善，心肌组织炎症细胞浸润减少，致炎因子表达减少(P0．05)。结论：地尔硫卓治疗AMI可以有效 改善左心功能和心室重塑，其机制可能与减弱心肌炎症细胞浸润和致炎因子表达有关。 [关键词] 急性心肌梗死；心室重塑；地尔硫卓；细胞因子 Study on intervention of the acute inflammatory responses following myocardial infarction by diltiazem Ying，LIAO 一Hua，CHENG Xiang．Department ofCardiology，Union Hospital，Huazhong Unive~ity ofScience and Technology，Wuhan 430022，China [Abstract] Objective：To explore the effects of dihiazem on ventricular remodeling and inflammation in rat heart following acute myocardial infarction(AMI)．Methods：The model of AMI rats was randomly divided into diltiazem group(D group)and control group (AMI group)，besides another group of sham operation(S group)．The data of ejection fraction(EF)and the left ventricular mass (LVM)were examined with echocardiography，and leukocyte infiltration in situ was analyzed on the HE staining slices，with the expres— sion of proinflammatory cytokines(IL—II3，IL-6，TNF— )detected by RT—PCR at ld，3d，1w，2w and 4w intervals after AMI．Results：The results from echocardiography showed that EF increased(73．7．4-3．1％vs 61．0．4-2．6％)and LVM decreased(0．81．4-0．12g vs 0．92± 0．12g)，both significantly in D group at4w，compared with those ofthe AMI group(PO．05)．Leukocyte infiltration and the expression 0fIL一1B，IL-6，and TNF— were remarkably lowered in D group(P0．05)，ofwhich IL一1p was 1．578±0．031 vs 0．546±0．029 at 4w．IL-6 was 0．952 4-0．012 vs 0．532 4-0．O11 at 2w，and TNF— was 1．001±0．006 vs 0．526±0．009 at 4w．Conclusion：Diltiazem could improve the left ventrieular function and att