善宁对大肠癌细胞体外抑制作用探讨.pdfVIP

肿瘤防治研究2007年第34卷第12期 ·949· 善宁对大肠癌细胞体外抑制作用探讨 李太原 ，张海涛 ，端木尽忠 Exploration of Inhibitory Effect of Sandostain to Colorectal Cancer Cell in vitro Li Tai-yuan ，ZHANG Hai—tao2，DUANMU Jin-zhong2 1．Tong1 l Medical College of Huazhong University of Science and Technology，Wuhan 430030，Chi— na；2．The First Affiliated Hospital 0f Nanchang University Abstract：0bjective In order to provide the experiment evidence of the endocrinotherapy for the patients with colorectal cancer，the inhibitions of the Sandostain to colorectal cancer cell were detected in vitro． Methods The sensitivity of Sandostain to 54 colorectal cancer cases were detected by MI T assay in vitro．Those patients were divided into various groups according to their sex，age，site，Dukes stages and the expression of p53．The sensitivity difference was analyzed by statistics in various groups．Results Sixteen cases were sensitive to Sandostain in 54 patients with colorectal cancer．The rate of sensitivity was 29．6 ．There were no relationship between the sensitivities of Sandostain to colorectal cancer cells and their sex，sites，Dukes stages as well as the expression of p53 protein，but the sensitivity of San— dostain was higher in the young group(≤60 y)than in the old group(6()y)．Conclusion Sandostain COuld inhibit cells proliferation in the most part of patients with colorectal cancer，the inhibitory effect of sandostain was stronger in young patients thain in the over 60y patients． Key words：Colorectal tumors；M r assay；In vitro；Gastrointestinal hormone；Drug sensitivity test 摘 要：目的 探讨生长抑素(善宁)对体外培养的大肠癌细胞的抑制作用，为大肠癌的内分泌治疗提 供实验依据。方法 采用MTT法体外检测善宁对54例大肠癌细胞的抑制率。依其性别、年龄、部位、 Dukes分期及p53的表达将其分组，统计分析各组病例的敏感药物的差异。结果 54例大肠癌病人中 有16例对善宁敏感，敏感率为29．6 。善宁敏感性与性别、部位、Dukes分期及p53蛋白的表达无关 ， 但与年龄有关，6O岁以下组(敏感率50 )较60岁以上组(敏感率13．3 )敏感，P0．01。结论 善宁 在体外对部分大肠癌患者癌细胞有抑制作用，对年轻患者大肠癌细胞抑制作用大于6()岁以上患者。 关键词：大肠癌 ；MTT法 ；体外 ；胃肠激素 ；药物敏感试验 中图分类号：R735．3 4 文献标识码：A 文章编号：1000