应用蛋白质芯片筛选壮族单纯性室间隔缺损血清标志蛋白.pdfVIP

广 西 医 科 大 学 学 报 JOURNALOF GUANGXI MEDICAL UNIVERSITY 2007 Dec；24(6) · 832 · SI 应用蛋白质芯片筛选壮族单纯性室间隔缺损血清标志蛋白 李永红 仇小强 曾小云 (广西壮族自治区疾病预防控制中心 南宁 530021) 摘要 目的：应用蛋白质芯片技术筛选壮族单纯性室间隔缺损(VSD)血清标志蛋白。方法：用表面增强激光解吸、电离飞行时 间质谱技术和CM10芯片检测25例壮族单纯性VSD患儿与36例儿科常见病患儿血清，用Ciphergen公司的Biomark Wizard 软件分析数据。结果：有12个蛋白具有统计学差异，其中质荷比(m／Z)6 640．784、8 5 70．1 54、8 698．2 78、2 244．582、 5 321．868、6 441．972和7 973．31O 7个蛋白在VsD患儿血清高表达，质荷比(m／z)2 213．579、4 604．572、2 142．465、9 198．582 和2 169．264 5个蛋白下调。结论：蛋白质芯片技术是发现与筛选室间隔缺损血清标志蛋白的快速、有效的蛋白质组学工具。 关键词 室间隔缺损；标志蛋白；蛋白质芯片技术；蛋白质组学 中国图书资料分类法分类号 R541．1；R34 SCREENING SERUM PRoTEIN MARKERS IN ZHUANG NATIoNALITY PATIENTS WITH SIMPLE VENTRICULAR SEPTAL DEFECT WITH PRoTEINCHIP TECHNoLoGY Li Yonghong，Qiu Xiaoqiang，Zeng Xiaoyun．(Guangxi Center for Disease Prevention and Control，Nan— ning 53002 1 China) Abstract 0bjective：Using ProteinChip technology to screen serum protein markers in patients with Simple Ventricular Septal Defect(VSD)．Methods：Serum samples from 25 Zhuang simple VSD patients and 36 patients with common pediatric diseases were detected by surface—enhanced laser desorption／ionization time- of-flight mass spectrometry(SELDI—TOF MS)technology and CM1 0 ProteinChip．Data were analyzed by Biomark Wizard software(Ciphergen Inc．)．Result：Serum proteins were significantly different between VsD and control group．Mass to charge ratio(m／z)6 640．784，8 570．154，8 698．278，2 244．582，5 321．868， 6 441．972 and 7 973．310 were of high expression in serum of VSD，and m／z 2 213．579，4 604．572， 2 142．465，9 198．582 and 2 169．264 were of lower expression in serum of VSD．Conclusion：ProteinChip technology is a quick and effective tool for proteomics，and it is capable of discovering and screening serum protein markers of VSD． Key words ventric