三种高渗溶液对蛙坐骨神经干动作电位的幅值及速度的影响论文.docVIP

　　三种高渗溶液对蛙坐骨神经干动作电位的幅值及速度的影响论文 黄晓丽，郑惠珍，黄钿生，黄秀玲，何泽江，刘永锋，吴淑芬，梁洪英 【关键词】 高渗溶液；双相动作电位；坐骨神经；蛙 【摘要】目的：观察葡萄糖、甘露醇、山梨醇3种高渗溶液对蛙坐骨神经干复合动作电位的幅度及传导速度的影响。方法：用青蛙制备蛙坐骨神经胫腓神经标本.freelotic dextroglucose,.freelannitol and sorbitol solutions on the amplitude and conduction velocity of pound action potential of the sciatic nerve of frog. Methods: 29 isolated sciatictibial peroneal nerve cord from frogs ly divided into four groups: control group (basic line group), hyperosmotic dextroglucose group (subgroups at concentrations of 10%，20% and 40% ), hyperosmotic mannitol group (subgroups at concentrations of 10% and 20%) and hyperosmotic sorbitol group (subgroups at concentrations of 10%，20% and 40%). MedLab biosignal collect and analysis system ployed to record pound biphasic action potential of nerve cords and to measure their conduction velocity. Results: pared plitude and conduction velocity of biphasic action potential otic dextroglucose subgroups (concentrations at 20% and 40%), hyperosmotic mannitol subgroup (concentration at 20%) and hyperosmotic sorbitol subgroups (concentrations at 20% and 40%) in a time dependent manner. 10% of hyperosmotic dextroglucose, hyperosmotic mannitol and hyperosmotic sorbitol slightly increase the amplitudes and mildly sloannitol and sorbital solution may inhibit the amplitude and conduction velocity of pound action potential of the sciatic nerve of frog, in后，再置于神经标本屏蔽盒内，中枢端距离刺激电极（S1）3mm。在神经干上放置2对记录电极（R1、R1′和R2、R2′），此时屏蔽盒R1到R2的长度为距离d；动作电位从R1传导到R2的时间为时间t。启动MedLab生物信号采集处理系统，设置实验参数为：记忆示波，刺激器触发，采样间隔25μs，放大倍数×200，全通滤波1kHz，主周期刺激，脉冲数1，波宽0.1 ms，延时10 ms，间隔60 ms，用70%的最大刺激强度作为刺激强度，记录神经干动作电位的幅值（A）与时程（t），按公式v＝ d/t（m/s）计算出动作电位传导速度。 每次实验时，将初始记录和测量到的动作电位幅值与传导速度定为动作电位幅值和传导速度的基础值（0 min），而后取出神经按实验分组分别放入任氏液和相应浓度的葡萄糖、甘露醇、山梨醇溶液中浸泡3～4 min后，置于神经标本屏蔽盒原来的位置上，每隔5min记录一次动作电位，连续观察60 min。期间于15 min、30 min和45 min 3个时刻分别滴入相应溶液湿润神经，再用滤纸轻轻吸干屏蔽盒内液体。 1.3 实验分组 实验分为：(1)葡萄糖组：下分3个浓度小组即质量分数为10%葡萄糖组、20%葡萄糖组和40%葡萄糖组；(2)甘露醇组：下分2个浓度小组即质量分数为10%甘露醇组和20%甘露醇组；(3)山梨醇组：下分3个浓度小组即质量分数为10%山梨醇组、20%山梨