药物干预逆转犬房颤心房电重构及神经重构.pdfVIP

新疆医科大学医学硕士学位论文 Revasal function of medication on Electrical Remodeling and Nerve Sprouting of Atrium in Canine Models of Pacing-induced Atrial Fibrillation Postgraduate: Zhang Yan supervisor:Pro.Hou Yue-mei Abstract Part One Electrical remodeling on atrium after 48 hours atrial tachy-pacing stop in Canine Models of Pacing-induced Atrial Fibrillation Objective:To examine the reversibility of the changes in electrophysiological remodeling on atrium in dogs with paroxysmal atrial fibrillation.Methods:Twenty-six adult mongel dogs were randomly divided into 3 groups randomly,all of 26 dogs were anesthetized and intubated with a tracheal tube. 1. 48 hours pacing group(n=8):high-speed paced at a rate of 600 bpm.2. 24 hours reversing group(n=10): monitoring 24 hours after ATP stoped. Sham group(n=8): tracheal tube was implanted and there were no interventions. The parameters reflecting the change of atrial electrophysiology properties were observed , including ERP, CV, WL, adaptation of ERP, inducibility of atrial fibrillation. at 0 h,48 h, 52 h,60 h,66 h, 72 h. The current of L-type calcium channel of atrial muscle cell was recorded by patch clamp technique in the whole cell mode. Results:The ERP ,CV,WL, adaptation of ERP of the Sinus Rhythm group is less than those of the Sham group at 48 hour. After converted to sinus rhythm, the ERP, adaptation of ERP of Sinus Rhythm group recovered to the baseline.But CV,WL remained less than baseline. The density of ICa-L current was significantly decreased in 48 hours in atrail tachypacing group.After pacing stop 24 hours, The densi