缺氧诱导因子-1α重组过表达质粒pcDNA3.1-HIF-1α的构建及其在前列腺癌中的作用及意义.pdfVIP

was constructed successfully;Both the pcDNA3.1-HIF-1α、RNA interference vector pll3.7-siHIF-1α were transfected into logarithmic growth phase prostate cancer PC-3,the transfection rate were respectively (49.24 ±2.56)% 、(42.36 ±2.62)%,the expression of HIF-1α mRNA was not increased in the over-expression cell compare to the other prostate cancer cells ，but the protein was increased significantly,while both the mRNA and protein of HIF-1α were decreased in the interference cell,the same as other factors associated with HIF-1α except for VEGF ；The ability of proliferation was enhanced and the invasion cell number was increased in the over-expression by MTT and transwell chamber. Conclusion The recombinant plasmid of HIF-1α was successfully constructed by RT-PCR,and the proliferation and invasion of prostate cancer were enhanced in the over-expression of HIF-1α,showed that HIF-1α has close relationship with the development and invasion metastasis of prostate cancer,revealed that HIF-1α may be an important factor in the pathogenesis of prostate cancer,which provide a new foundation for the pathogenesis research of prostate cancer,and genic therapy targeted at HIF-1α may be a new track for the treatment of prostate cancer. Key Word:Recombinant plasmid ；HIF-1α；Prostate Cancer Cell PC-3 ；Effect ； Significances; 8 英文缩略词表 缩略语 英文全称 中文注释 HIF-1α hypoxia-inducible factor-1α 缺氧诱导因子-1α HIF-1β hypoxia-inducible factor-1β 缺氧诱导因子-1β VEGF vascular endothelial growth factor 血管内皮生长因子 MMP-2 matrix metalloproteinase-2 基质金属蛋白