缺血再灌注对肝癌细胞多药耐药表型的影响.pdfVIP

Effect of ischemia reperfusion on multidrug resistancephenotype of hepatocellular carcinoma cells Abstract Objective: This experiment explores ischemia-reperfusion results in changes of microenvironment effect on liver cancer cell multidrug-resistance by simulation of human hepatocellular carcinoma cell line (HepG2) and Adriamycin resistant cell line of human hepatocellular carcinoma (HepG2/ADM) during procedure of ischemia-reperfusion, providing the basis for preliminary study of multidrug resistance to further explore the new way to reverse liver cancer cells. Method ：The human hepatocellular carcinoma cell line HepG2 and Adriamycin resistant cell line of human hepatocellular carcinoma HepG2/ADM were cultured to logarithmic growth phase, two cell lines were set up perfusion group (HepG2 perfusion group: HC group; HepG2/ADM perfusion group: HAC group) and ischemia-reperfusion group (HepG2 ischemia reperfusion group: HR group; HepG2/ADM ischemia reperfusion group: HAR group), in which perfusion group with reperfusion 8 hours; ischemic-reperfusion group with ischemic solution for 4 hours, the reperfusion fluid for 4 hours, simulated hepatoma cells during ischemia reperfusion.Inverted microscope was used to observe the morphological changes of the cells in perfusion group and ischemia reperfusion group ，Expression of reverse transcriptase semi quantitative 5 PCR detection control group and ischemia reperfusion group multidrug resistance gene-1(MDR1) and multidrug resistance protein-2 (MRP2) mRNA ， Western blot was used to detect the protein expression of P-glycoprotein( P-gp ) and lung resist