急性早幼粒细胞白血病发病期与缓解期MTHFR mRNA表达差异研究.pdfVIP

第50 卷第5 期 哈尔滨医科大学学报 VoLSO ,No.S 440 2016 年 10 月 JOURNAL OF HARBIN MEDICAL UNIVERSITY Oct. , 2016 急性早幼粒细胞白血病发病期与缓解期 MTHFR mRNA 表达差异研究 汤海蒂1 ，叶向梅2\杨宝义3 (1.哈尔滨医科大学附属第一医院实验诊断学教研室;2. 血液肿瘤中心实验室;3. 黑龙江省中医药大学 附属第一医院脑外科，黑龙江哈尔滨 15α)()1 ) [摘要] 副崎 研究 MTHFR 基因在急性早幼粒细胞白血病急性期及缓解期表达差异。方旅 选取急 性早幼粒细胞白血病患者269 例，其中急性期 183 例，缓解期86 例，采集骨髓血2 mL，通过实时定量RT­ PCR 技术检测 MTHFR mRNA 表达水平。结.. 急性早幼粒细胞白血病急性期 MTHFR 表达水平显著 高于缓解期(P 0. 05) 。锦俗 MTHFR 基因高表达可能是急性髓系白血病M3 型的发病原因之一。 [关键词] 急性早幼粒细胞白血病;四氢叶酸还原酶;基因表达;甲基化 [中图分类号]R733.71 [文献标识码)A [文章编号]1000-19臼(2016)05 -0440 -03 Study on the relationsbip between the expression of MTHFR mRNA and the arsenic methylation 1 2 3 TANG Hai-di , YE Xiang-mei , YANG Bao-yi (1.TheLαboratory Diagnosis of Teαching and Research Section; 2. The Center Laborαω， ry of He- matowgical Neoplω阳， The First Affiliated Hospital of Harbin Medical University; 3. Depαrtment of Cerebral Surgery , The First Ajj号liated Hospital ofHeilongjiang University of Chinese Medicine , Hαrbin Öω01 ， China) Abstract: Objective To observe expression differences of the MTHFR mRNA in patients wi出 acute promyelocytic leukemia during onset phase and remission. Methods Two hundred and sixty-nine patients wÍth acute promyelocytic leukemia were selected , including 183 onset phase and 86 remission. Bone marrow samples were collected and lymphocytes of bone marrow were separated to detect expression of MTHFR mRNA by using RT-PCR. Results MTHFR mRNA expression in the patients during onset phase was higher compared with those in the patients during remission (P O. 05). Conclusion Higher expression of MTHFR mRNA may lead t。 由e occurrence of acute promyelocytic leukemia. Key words: acute promyelocytic leukemia; methylenete