M细胞模型及其在生物大分子药物口服递药研究中的应用.pdfVIP

药学学报 Acta Pharmaceutica Sinica 2011, 46 (12): 1429?1435 · 1429 · M 细胞模型及其在生物大分子药物口服递药研究中的应用 李亨芬, 邹 金, 白如玉, 邢永梅, 聂金梅, 刁 勇* (教育部分子药物工程研究中心, 华侨大学分子药物学研究所, 福建 泉州 362021) 摘要: 如何克服胃肠道吸收屏障是蛋白质、多肽和核酸等生物大分子药物口服给药面临的重大挑战。M 细 胞是胃肠道上皮细胞内一种特殊的抗原摄取细胞, 具有高内吞和低降解特征, 可能对生物大分子药物的口服吸 收发挥重要作用。M 细胞体外共培养系统的开发, 以及在其基础上建立的体外模型, 促进了对 M 细胞本身以及 大分子药物口服给药系统的研究。本文综述了 M 细胞特殊的结构、功能、形成及生物标志特点, 讨论了 M 细胞 体外模型在开发生物活性大分子药物口服给药系统的应用。 关键词: M 细胞; 体外模型; 大分子药物; 口服递药系统; 口服疫苗 中图分类号: R943 文献标识码: A 文章编号: 0513-4870 (2011) 12-1429-07 M cell in vitro model and its application in oral delivery of macromolecular drugs LI Heng-fen, ZOU Jin, BAI Ru-yu, XING Yong-mei, NIE Jin-mei, DIAO Yong* (Engineering Research Center of Molecular Medicine, Ministry of Education, Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China) Abstract: The oral administration of bioactive macromolecular drugs such as proteins, peptides and nucleic acids represents unprecedented challenges from the drug delivery point of view. One key consideration is how to overcome the gastrointestinal tract absorption barrier. Recent studies suggest that microfold cell (M cell), a kind of specialized antigen-sampling epithelial cell which is characterized by a high endocytic rate and low degradation ability, may play an important role in macromolecule oral absorption. The development of an in vitro M cell coculture system and its modified models greatly advanced the study of M cells and the development of oral d