金花菌黄色素的稳定性及其抗氧化活性研究.PDFVIP

微生物学通报 Nov. 20, 2013, 40(11): 2030?2036 Microbiology China ? 2013 by Institute of Microbiology, CAS tongbao@ 基金项目：陕西省科技厅统筹项目(No. 2011KTCQ03-08) *通讯作者：Tel: 86-29 : lujl@ 收稿日期：2012-12-03; 接受日期：2013-04-02 研究报告 金花菌黄色素的稳定性及其抗氧化活性研究 李玉婷 吕嘉枥* (陕西科技大学 生命科学与工程学院 陕西 西安 710021) 摘 要: 【目的】评价茯砖茶金花菌黄色素的稳定性和抗氧化性能。【方法】以色素保存 率为指标, 研究温度、光照和 pH 对黄色素稳定性的影响。并对还原能力、DPPH·清除能 力和 H2O2 诱导的红细胞溶血抑制能力进行测定, 考察黄色素的抗氧化性能。【结果】该色 素在低温下保存 7 d 保存率高于 98.88%, 温度升高, 保存率降低; 光照尤其是太阳光对其 稳定性影响较大; 黄色素在 pH 2?10 较稳定, pH 12 时色素保存率仅为 46.22%。低浓度黄 色素具有明显高于维生素 E (VE)的还原能力、DPPH·清除率和溶血抑制率, 其中 DPPH·的 EC50 低于 50 mg/L。【结论】金花菌黄色素对热、酸和弱碱较稳定, 对强碱和太阳光极不 稳定, 并有优良的抗氧化能力, 研究和开发前景良好。 关键词: 冠突散囊菌, 色素, 稳定性, 抗氧化性 Study on stability and antioxidant activity of the yellow pigments from Eurotium cristatum LI Yu-Ting Lü Jia-Li* (College of Life Science Engineering, Shaanxi University of Science Technology, Xi’an, Shaanxi 710021, China) Abstract: [Objective] Stability and antioxidant activity of the yellow pigments from Eurotium cristatum were evaluated. [Methods] Taking preservation rate of the pigments as an indicator, the effect of different temperature, light and pH on the stability were assessed. Antioxidant ac- tivity of the yellow pigments was investigated by three methods: reducibility, DPPH· scav- enging activity and inhibition of erythrocyte hemolysis induced by H2O2. [Results] The results 李玉婷等: 金花菌黄色素的稳定性及其抗氧化活性研究 2031 /wswxtbcn of stability test showed that preservation rate of the pigments was more than 98.88% after low-temperature treatment for 7 days and declined as the temperature increasing. Light, espe- cially sunlight, resulted in an obvious loss of stability of the pigments. Better stability of the pigments was observed in the range of pH 2?10. However, preservation rate was lower than 46.22% at pH 12. At low concentration the pigments had higher reducibility, DPPH· scaveng- ing activity and