2240例体检中心女性HPV感染状况及基因型分析.docVIP

2240例体检中心女性HPV感染状况及基因型分析 　　摘要：目的 调查和分析杭州市萧山区浙江萧山医院体检中心女性中人乳头瘤病毒（H PV） 感染以及基因亚型分布状况，为预防HPV感染和宫颈癌防治提供依据。方法 利用DNA杂交技术检测本院体检中心女性标本，并进行基因分型。结果 在2240例样本中HPV感染者311例，感染率为13.88%，其中高危亚型检出232例， 占感染者的74.59%，低危亚型54例，占17.36%；同时感染高危亚型和低危亚型的25例，占8.04%；单一感染254例，占感染者的81.67%，两重感染42例，占13.50%，≥三重的多重感染15例，占4.82%。23个亚型中52型最多，其次为43和16型，各年龄段感染率无显著性差异（P0.05）。结论 萧山地区健康体检女性HPV总感染率为13.88%， 单一感染为本地区常见类型，以HPV52、43和16为主要感染亚型。 　　关键词：人乳头瘤病毒；基因分型；DNA杂交 　　Analysis of Human Papilloma Virus Infection State and Genotypes of 2240 Clients in Centre of Physical Examination 　　ZHONG Rui， WANG Min 　　（Zhejiang Xiaoshan Hospital， Hangzhou 311201， Zhejiang，China） 　　Abstract：Objective To study the infection status and genotypes of 23 genotypes of human papillomavirus （ HPV） in Hangzhou City，Xiaoshan district，and to provide theoretical basis for preventing and treating uterine cervical cancer. Methods 2240 samples were detected for HPV genotypes by using DNA hybridization technique. Results 311 cases were HPV， with the positive rate of 13.88%，232 cases belonged to high-risk type only， with the ratio of 74.59% in HPV positive cases， 54（17.36%） cases for low-risk type， 25（8.04%） cases were mixed with high-risk type and low-risk type. Subtype HPV52 occupied the first of all high-risk types， followed by subtype HPV43 and HPV16. 254 cases were the single HPV genotype， with the ratio of 81.67% in HPV positive cases， 42（13.50%） cases were double HPV genotypes， and 15（4.82%） cases were more than three. By the comparison in accordence with the disparity in age section，masculine gender rate in the groups with various age has no significance difference after the chi-square test. Conclusion Healthy check-up women in Xiaoshan area HPV the total infection rate was13.88%. Single genotype is the most common HPV infection type，and HPV52、HPV43 and HPV16 are the major subtypes. 　　Key words：Human papillomavirus；Genotype；DNA hybridization 　　宫颈癌作为一种恶性肿瘤发病率非常高，仅次于乳腺癌的发病，在女性癌症中排列第2位，对女性的身心造成了严重的伤害。Zur Hausen等在20世纪70年代就从宫颈癌的癌变组