02免疫球蛋白幻灯片.pptVIP

Outline Introduction Structure of Immunoglobulin Function of Immunoglobulin Characteristics and Functions of 5 Ig Classes Monoclonal Antibody 1.Antibody (Ab) 2. Immunoglobulin (Ig) A. Conception B. Expression of Ig A.Joining chain  B.Secretory piece (SP) or  secretory component (SC) Ⅳ.Characteristics and  Functions of 5 Ig Classes Immunoglobulin Superfamily 1.Conception and structure of IgSF * * Chapter 3 (conception of Ab and Ig) neutralize exotoxin antitoxin agglutinin agglutinate Ag Ab Discovery of antibody specific substance (in serum) animal bacteria 含有抗体的血清 称为免疫血清 Ⅰ. Introduction Ag → B cell ↓ plasma cell ↓ globulin ?Conception of Ab ?Physicochemical properties of Ab ? can combine with corresponding Ag ? immune functions (antibody) 抗体是B细胞受抗原刺激后,增殖分化为浆细胞所产生的一种能与相应抗原结合、具有免疫功能的球蛋白 球蛋白 正常人血清电泳分离图 ?gamma-globulin ?heterogenicity (异质性) 具有抗体活性或化学结构与抗体相似的球蛋白统称为免疫球蛋白(Ig) ?Secreted Ig (SIg) ?Membrane Ig (mIg ) 1.Basic structure of Ig * tetrapeptide chain structure Ⅱ. Structure of Ig * monomer of Ig (appear Y shaped) ---basic unit of Ig molecule (Ag-biding site) L chain H chain (?,?,?,?,?) V region C region ? H2L2 ? hinge region ? 2 region κ/λ (2:1) Monomer of Ig FR2 FR1 HVR (hyper-variable region) CDR(complementarity determining region) HVR1/CDR1 HVR2/CDR2 HVR3/CDR3 Ag-biding site ---made up of VH and VL (HVR/CDR) FR3 FR4 FR (framework region) 2. Domain of Ig VH/VL --- Ag-binding site (HVR/CDR) CH2--- complement C1q binding site (CH3 of IgM) CH1/CL ---genetic marker of allotype CH3,4--- binding to FcR on surface of cells(CH2, 3 of IgE binding to Fc?R) 19 IgG 3.Hydrolytic fragment of Ig 2 Fab + 1Fc 1 F(ab’)2 +pFc’ (papain) (pepsin) C.Secretory IgA: (H2L2)2-SC-J 4. Joining chain ＆ secretory piece n(H2L2) n(monomer) ---保护SIgA并介导IgA分泌到粘膜表面 J chain (H2L2)n J polymer (H2L2)2 J